开放科学(资源服务)标识码(OSID) Effect of Tanshinone ⅡA on abnormal integration of granule cells in the dentate gyrus of mice with chronic epilepsy / 西安交通大学学报(医学版)

【Objective】 To clarify the role and molecular mechanism of Tanshinone ⅡA (TanⅡA) in the pathological integration of granule cells in the dentate gyrus (DG) by using the mouse model of temporal lobe epilepsy (TLE). 【Methods】 Status epilepticus (SE) was induced in the mice with pilocarpine and treated with TanⅡA 5 mg/kg. After two months, Morris water maze was used to examine the spatial learning and memory ability and video surveillance was used to monitor spontaneous seizures. The DG was removed for staining of Timm, Prox-1, DCX and SynⅠ. PTEN, p-AKT, and p-S6 expressions were observed by Western blotting. 【Results】 TanⅡA decreased Timm score, SynⅠ, PSD-95 and pS6 levels, and increased the level of PTEN in the DG, and attenuated the formation of mossy fiber sproutings and basal dendrites of the granule cells. Video surveillance showed that TanⅡA reduced the frequency of Racine’ grade 5 seizures. 【Conclusion】 TanⅡA can effectively attenuate the abnormal integration of the granule cells in the DG by regulating PTEN/AKT/mTOR pathway and thus plays an anti-epileptic role.

Construction of an acute toxoplasma encephalitis mouse model by the stereotaxic surgery with RH toxoplasma trophozoites / 西安交通大学学报(医学版)

【Objective】 To construct an acute toxoplasma encephalitis mouse model by observing the pathological changes in the hippocampus of mice infected with Toxoplasma gondii strain RH. 【Methods】 The quantitative RH Toxoplasma gondii (100, 500, and 1 000 trophozoites) were injected into the hippocampal CA1 region of mice by the stereotaxic surgery; the survival status of mice was observed. Giemsa staining was used to observe the changes of toxoplasma in mouse ascites and brain tissue homogenates. Nissl staining and HE staining were used to observe the pathological changes of hippocampal nerve tissue. The distribution of Toxoplasma gondii in brain tissue was observed by immunohistochemical ABC method. 【Results】 The RH Toxoplasma gondii infected mice showed obvious symptoms such as arched back, bristling hair, abdominal distension, subtle tremor and hemiplegia on the fourth day of infection. The survival of mice in 100 trophozoites group was longer, no trophozoites of Toxoplasma gondii were found in ascites, a few pseudocysts were found in brain tissue homogenates after infected for 96 hours, and more trophozoites were found after death. Nysl staining and HE staining showed more tissue necrosis foci and loss of nerve cells in CA1 area after infected 144 h. The injury aggravated with the prolongation of infection time. Toxoplasma trophozoites were found in ascites and brain homogenates of mice in 500 and 1000 trophozoites groups. Nissl staining revealed neuronal loss and massive necrosis in the hippocampus. HE staining showed necrosis and inflammatory cell infiltration. The brain tissue injury significantly aggravated compared with 100 trophozoites group. The distribution of Toxoplasma gondii in the necrotic foci was confirmed by immunohistochemistry. 【Conclusion】 The survival of 100 trophozoite mice infected with Toxoplasma gondii strain RH was longer, and the pathological changes of brain tissue gradually aggravated. The damage was relatively confined to the brain tissue, and the mice showed typical symptoms of toxoplasma encephalitis. Therefore, the mouse model of acute toxoplasma encephalitis can be constructed by localized infection of 100 toxoplasma trophozoites, which can lay a foundation for future research on the mechanism of toxoplasma injury to cranial nerves.

Huanglian Jiedutang Treats Acute Gouty Arthritis via NLRP3 Inflammasome and TLR4/NF-κB Signaling Pathway / 中国实验方剂学杂志

ObjectiveTo observe the effect of Huanglian Jiedutang on the inflammatory injury in the mouse model of acute gouty arthritis （AGA） and to explore the mechanism of Huanglian Jiedutang in treating AGA. MethodForty male C57BL/6J mice were randomized into blank， model， colchicine （0.83 mg·kg-1）， and Huanglian Jiedutang （5 g·kg-1） groups. The mouse model of AGA was established by injecting monosodium urate （MSU） crystals into the ankle joint. The swelling degree of the right ankle joint of each mouse was measured every day for 7 days， and the pathological changes of the ankle joint were detected by hematoxylin-eosin （HE） staining after 7 days. The other 40 C57BL/6J mice were grouped as above. After 18 hours of modeling， the right ankle joint was collected， and real-time polymerase chain reaction was employed to measure the mRNA levels of interleukin （IL）-1β， tumor necrosis factor （TNF）-α， IL-6， NOD-like receptor protein 3 （NLRP3）， apoptosis-associated speck-like protein containing a CARD （ASC）， and Caspase-1. The expression levels of IL-1β， TNF-α， and IL-6 were measured by the enzyme-linked immunosorbent assay. Western blot was employed to determine the protein levels of NLRP3 inflammasome， Toll-like receptor 4 （TLR4）， and nuclear factor-κB （NF-κB）. ResultCompared with the blank group， the model group showed swelling right ankle joint （P<0.01）， obvious foreign body granuloma in the ankle joint with inflammatory cell infiltration. After the treatment with Huanglian Jiedutang， the ankle joint swelling was relieved （P<0.05， P<0.01）， and the size of foreign body granuloma was reduced. Compared with the blank group， the model group showed up-regulated mRNA levels of IL-1β， TNF-α， and IL-6 in the ankle joint tissue （P<0.01）， up-regulated mRNA levels of NLRP3 and Caspase-1 in the NLRP3 inflammasome （P<0.05， P<0.01）， and up-regulated protein levels of NLRP3， Caspase-1， TLR4， and NF-κB （P<0.05， P<0.01）. Huanglian Jiedutang down-regulated the mRNA levels of IL-1β， TNF-α， IL-6， NLRP3， and Caspase-1 （P<0.05， P<0.01） and the protein levels of IL-1β， TNF-α， IL-6， NLRP3， Caspase-1， TLR4， and NF-κB （P<0.05 or P<0.01）. ConclusionInjecting MSU crystal resulted in local inflammatory injury of the joints in the mouse model of AGA. The treatment with Huanglian Jiedutang may alleviate the inflammatory injury by regulating the NLRP3 inflammasome and TLR4/NF-κB signaling pathway.

Fibrinogen gamma-chain mutation, p.Ile171His, leads to hereditary hypofibrinogenemia / 中华内科杂志

Objective:To explore the clinical phenotype and genotype of a family with hereditary hypofibrinogenemia.Methods:Activated partial thrombin time (APTT), prothrombin time (PT),thrombin time (TT) and thrombelastogram (TEG) were tested in all family members. Fibrinogen activity and antigen were detected by Clauss method and immunoturbidimetric method respectively. All exons and flanking sequences of fibrinogen FGA,FGB,FGG genes were analyzed by PCR, and the products were subjected to Sanger sequencing.Results:The proband represented prolonged PT and TT, low Fg activity and antigen, elevated K value and decreased Angle value in TEG. Other family members reported similar changes including proband′s father,daughter and son, and his elder brother and his niece. Exon 5 c.510_512 of FGG gene in the proband revealed a minor deletion mutation.Conclusion:The novel heterozygous missense mutation of exon 5 c.510_512del (Gln170_Ile171 del ins His) of FGG gene is the molecular mechanism that leads to hereditary hypofibrinogenemia in this family.

Huanglian Jiedutang Regulates Inflammatory Immunity to Relieve Rheumatoid Arthritis / 中国实验方剂学杂志

ObjectiveTo observe the effects of Huanglian Jiedutang on pathological and immune damage in collagen-induced arthritis （CIA） model mice， and to explore the possible mechanism of Huanglian Jiedutang in relieving rheumatoid arthritis. MethodTwenty-four DBA/1 mice were randomly divided into normal group， model group， methotrexate group and Huanglian Jiedutang group， with six mice in each group. The CIA mice model were established using type Ⅱ collagen induction. The administration groups were respectively treated with Huanglian Jiedutang （5 g·kg-1） and methotrexate （0.5 mg·kg-1）. The joint swelling symptoms of the mice were observed， and the arthritis index was scored every 3 days. Flow cytometry was employed to detect granulocytes， monocytes， and T lymphocytes in peripheral blood. The expression of inflammatory cytokines in joint was determined by real-time polymerase chain reaction （Real-time PCR）. The ankle joint was scanned by micro-computed tomography （Micro-CT）， and the histopathological changes were observed through hematoxylin-eosin （HE） staining. ResultCompared with the normal group， the modeling led to joint swelling， elevated the joint index score （P<0.05）， increased the proportion of granulocytes （P<0.05） and decreased that of monocytes and T lymphocytes （P<0.01） in peripheral blood， and raised the neutrophil-to-lymphocyte ratio （NLR） （P<0.01）. Further， it up-regulated the expression of inflammatory cytokines such as tumor necrosis factor-α （TNF-α）， interleukin （IL）-1β， and IL-6 in joint （P<0.01）. Micro-CT showed obvious bone destruction in the ankle joint， and pathological examination revealed the infiltration of a large number of inflammatory cells and the synovial hyperplasia of joint tissue. Compared with the model group， Huanglian Jiedutang alleviated the symptoms of joint swelling， lowered the score of arthritis index （P<0.05）， increased the proportion of T lymphocytes and lowered NLR （P<0.01）. Moreover， it down-regulated the expression levels of TNF-α， IL-1β， and IL-6 in joint （P<0.01） and alleviated the bone destruction and pathological injury of joint tissue. ConclusionType Ⅱ collagen caused systemic and local inflammatory immune damage in CIA mice. Huanglian Jiedutang alleviates such injury， especially for that in local joint， thereby inhibiting joint injury and bone destruction in CIA mice.

Clinical and genetic analysis of a novel CHD4 gene variant in a Chinese patient with Sifrim-Hitz-Weiss syndrome / 中华医学遗传学杂志

OBJECTIVE@#To explore the genotype-phenotype correlation of a case with Sifrim-Hitz-Weiss syndrome (SIHIWES) caused by a novel CHD4 gene variant.@*METHODS@#Genomic DNA was extracted from peripheral blood samples of the patient and her parents. Whole-exome sequencing (WES) was carried out for the patient.Suspected variant was verified by Sanger sequencing.@*RESULTS@#The proband, a 2-year-old Chinese girl, presented with global developmental delay, intellectual disability, distinctive facial features and multiple congenital anomalies. Her prenatal manifestations included increased nuchal thickness, cranial and facial anomalies, and decreased fetal movement. WES has identified a novel variant in the CHD4 gene, namely NM_001273:c.2989C>G (p.Leu997Val) (GRCh37/hg19).Comparison of her phenotype with previously reported SIHIWES cases suggested that our patient's prenatal presentations were unreported before, with novel features including funduscopic anomaly, facial dysmorphisms such as asymmetrical ears, drooping eyelid, long philtrum and downturned mouth.@*CONCLUSION@#Above findings have expanded the mutational spectrum of the CHD4 gene and revealed novel phenotypes in Chinese patients with SIHIWES.

Experimental study on relative deviation between display value and measured value of entrance surface dose (ESD) in digital radiography and influencing factors / 中华放射医学与防护杂志

Objective:To compare the relative deviation between display value and measured value of entrance surface dose (ESD) at different tube voltages, source-to-image distances and phantom thicknesses, so as to provide the basis for the feasibility of using real-time ESD display value to evaluate the radiation dose and the suitability of exposure parameters.Methods:At two different tube voltages and source-to-image distances, the exposure experiments were carried out using three polyethylene models with surface area of 30 cm×30 cm and thickness of 5 cm, 10 cm and 20 cm, respectively. After each exposure, the ESD display values on DR equipment and the measured values on radiation dose detector were recorded. The Wilcoxon rank sum test was used to compare the difference between them, with the relative deviation of ESD display value calculated using formula.Results:At the same tube voltage and source-to-image distance, the ESD display values were not affected by the thickness of phantom, but the measured value changed with the thickness of the phantom. When the thickness of the phantom was 10 cm and 20 cm respectively, the ESD display value was smaller than the measured value and the difference was statistically significant ( Z=-2.201, -2.203, P<0.05). The thicker mold would lead to the larger relative deviation between display value and measured value. Only when the thickness of mold was 20 cm and source-to-image distance 100 cm, the relative deviation was greater than ±20%. Conclusions:The ESD display value on DR equipment can be used to evaluate the skin incident dose to the examinee, with necessary account taken of the influence of their body thickness.

Meta-analysis on prevalence of iron deficiency anemia in Chinese children aged 0-14 years from 2000 to 2020 / 中国学校卫生

Objective@#To systematically review the prevalence of iron-deficiency anemia among children aged 0-14 years in China from 2000 to 2020, and to provide a reference for prevention and controlling of IDA among Chinese children.@*Methods@#CNKI, CBM, WanFang Data, VIP databases, PubMed, Embase and Web of Science were electronically searched to collect crosssectional studies on the prevalence of iron deficiency anemia in children aged 0-14 years of China from 2000 to April 2020. Two reviewers independently conducted literature screening, methodological evaluation and data extraction, and used Stata 13.0 software to combine the data to estimate the prevalence. The Q test and I 2 statistics were used to evaluate the heterogeneity of studies. Begg and Egger test were used to evaluater.@*Results@#A total of 60 articles were included, including 122 771 children, among whom 28 693 were sick. Meta-analysis results showed that the total prevalence rate of children aged 0-14 years in China from 2000 to 2020 was 19.9%. The prevalence rate of girls (18.7%) was higher than that of boys (16.9%), and the difference was statistically significant(P<0.05). The prevalence rate was highest in infancy(30.3%), followed by in early childhood(16.7%). From 2006 to 2010, the prevalence rate of children was the highest(22.6%). In recent years, the prevalence rate of children with iron deficiency anemia was lower than before. Mild anemia was found in 88.7% of the children, and moderate or severe anemia was found in 11.3% of the children. The prevalence rate of children in western China was the highest, 31.9% and 28.3% respectively, and the incidence rate in east China(13.1%), south China (14.0%) and northeast China (16.6%) was relatively low. The prevalence rate of rural children (25.6%) was much higher than that of urban children(9.1%), especially in western rural areas.@*Conclusion@#The prevalence of iron-deficiency anemia among children aged 0-14 years in China from 2000 to 2020 is still high and the differences between different regions are significant, so more attention should be paid to the prevention and treatment of iron-deficiency anemia among infants and children in poor areas.

Identification of a de novo MAP2K1 gene variant in an affected patient with Cardio-facio-cutaneous syndrome / 中华医学遗传学杂志

OBJECTIVE@#To explore the genotype-phenotype correlation of Cardio-facio-cutaneous syndrome (CFCS) caused by MAP2K1 gene variants.@*METHODS@#Genomic DNA was extracted from peripheral blood sample from a child patient and his parents. Whole exome sequencing (WES) was carried out for the patient. Suspected variant was verified by Sanger sequencing.@*RESULTS@#The patient was a 1-year-8-month old Chinese male who manifested short stature, psychomotor retardation, relative macrocephaly, distinctive facial features, and congenital heart disease. WES test revealed a heterozygous missense c.389A>G (p.Tyr130Cys) variant in the MAP2K1 gene. Sanger sequencing has confirmed the variant as de novo. According to ACMG/AMP guidelines, the variant was classified as pathogenic.@*CONCLUSION@#Compared with previously reported CFCS cases due to MAP2K1 variants. The patient showed obvious behavioral problems, good appetite and tricuspid regurgitation, which may to be novel features for CFCS.

Cohort study on the relationship between women’s pre-pregnancy age, alanine aminotransferase, blood glucose level and premature birth / 公共卫生与预防医学

Objective　 To investigate the effect of pre-pregnancy age, alanine aminotransferase (ALT), blood glucose (GLU) level on premature birth. Methods Based on the free pre-marital health check-ups and pre-pregnancy eugenic health check-ups in Dongguan, the examination results of the subjects were obtained and the pregnancy outcomes were followed up. Logistic regression models were used to analyze the relationship between women’s pre-pregnancy age, ALT, and GLU levels and the incidence of premature birth. Results A total of 18 150 subjects were included in this study, and the overall incidence of premature birth was 6.06% (1,099/18,150). The women’s pre-pregnancy age 30 to 34 (RR=1.26, 95% CI:1.07～1.48), age>34 (RR=1.82, 95% CI:1.52～2.18), ALT≥40 U/L (RR=1.40, 95% CI: 1.09～1.80) and GLU>6.1 mmol/L(RR=1.74, 95% CI: 1.01～2.97) were the influencing factors of premature birth. Conclusion Older women, abnormal serum alanine aminotransferase level and hyperglycemia had an impact on the occurrence of premature birth, which has important guiding significance for health education of eugenics before pregnancy.

Practice of "Disease-Characteristics Nursing Quality Improvement" Program based on Kotter's change model / 中国实用护理杂志

Objective@#To explore the application effect of "Disease-Characteristics Nursing Quality Improvement" Program (DNQIP) based on Kotter's change model in continuous improvement of patients' satisfaction and nursing quality.@*Methods@#Applicate Kotter's Change Model to implement DNQIP among 61 wards. The differences of patients' satisfaction and nursing quality among 2012 were compared (DNQIP had not been carried out by the whole hospital), 2014 (DNQIP had been carried out by the whole 61 wards) and 2016 (the 5-year-strategy had been realized).@*Results@#The 3-year-scores of patients' satisfaction were(95.94±1.53), (98.18±0.69), (99.43±0.18) points and they were statistically significant (F=31 768.863, P=0.000).The 3-year-scores of nursing quality were (93.96±0.56), (98.56±0.43), (99.62±0.23) points and they were statistically significant (F=3 004.598, P=0.000). The sores of 2014 were statistically improved compared with 2012 and the sores of 2016 were statistically improved compared with 2014 (P<0.01).@*Conclusions@#DNQIP based on Kotter's change model is an effective method to improve the patients′ satisfaction and nursing quality.

Practice of"Disease-Characteristics Nursing Quality Improvement"Program based on Kotter's change model / 中国实用护理杂志

Objective To explore the application effect of "Disease-Characteristics Nursing Quality Improvement" Program (DNQIP) based on Kotter's change model in continuous improvement of patients' satisfaction and nursing quality. Methods Applicate Kotter's Change Model to implement DNQIP among 61 wards. The differences of patients' satisfaction and nursing quality among 2012 were compared (DNQIP had not been carried out by the whole hospital), 2014 (DNQIP had been carried out by the whole 61 wards) and 2016 (the 5-year-strategy had been realized). Results The 3-year-scores of patients' satisfaction were(95.94 ± 1.53), (98.18 ± 0.69), (99.43 ± 0.18) points and they were statistically significant (F=31 768.863,P=0.000).The 3-year-scores of nursing quality were (93.96 ± 0.56), (98.56 ± 0.43), (99.62 ± 0.23) points and they were statistically significant (F=3 004.598,P=0.000). The sores of 2014 were statistically improved compared with 2012 and the sores of 2016 were statistically improved compared with 2014 (P＜0.01). Conclusions DNQIP based on Kotter's change model is an effective method to improve the patients′satisfaction and nursing quality.

Association between serum biochemical parameters and transient ischemic attack / 中华老年心脑血管病杂志

Objective To study the association between H cy,hs-CRP,IMA and transient ischemic attack (TIA).Methods One hundred and twenty-six TIA patients were divided into low risk group (n=42),moderate risk group (n=43) and high risk group (n=43) according to their AB-CD2 score with 20 healthy subjects undergoing physical examinarion served as control group.Their clinical data were recorded and their serum Hcy,IMA and hs-CRP levels were compared.Results The serum levels of TC,TG,LDL,Hcy,IMA and hs-CRP were significantly higher while those of HDL were significantly lower in low risk group,moderate risk group and high risk group than in control group (P＜0.05),in moderate risk group and high risk group than in low risk group (P＜0.05),and in high risk group than in moderate risk group (P＜0.05).The serumlevels of Hcy,hs-CRP and IMA were positively associated with ABCD2 score in TIA patients (r=0.36,r =0.31,r =0.24,P＜0.05) but not associated with each other (P＞0.05).Multivariate logistic regression analysis showed that hyperlidemia and Hcy were the risk factors for TIA (P＜0.05,P＜0.01).Conclusion Serum Hcy,hs-CRP,IMA levels are positively associated with AB-CD2 score.Hyperlipidemia and Hcy are the risk factor for TIA.Measurement of serum Hcy,hsCRP,IMA levels is beneficial to the assessment of TIA.

Progresses of ultrasound-guided percutaneous microwave ablation in treatment of adenomyosis / 中国介入影像与治疗学

Adenomyosis is a frequently encountered disease with grave symptoms.There are many therapeutic approaches of internal medicine and surgery for adenomyosis in the clinic,while none of them is satisfactory.Ultrasoundguided percutaneous microwave ablation for adenomyosis is a newly emerged therapeutic method with many advantages,such as its minimally invasive procedure and approving therapeutic effect.The progresses of ultrasound-guided percutaneous microwave ablation in treatment of adenomyosis were reviewed in this article.

Influence of persistent concept based on strong concept on long-term hope level of patients with breast cancer surgery / 现代临床护理

Objective To investigate the effect of persistent concept based on strong concept on long-term hope of patients with breast cancer undergoing extended-concept nursing.Methods Two hundred and sixty-six patients with breast cancer were randomly divided into control group (n=62) and observation group (n=64) according to the discharge order.The routine health education and instruction were carried out plus strong concept of nursing intervention for three times during hospitalization.About 2 months after discharge the observation group was treated with the strong concept of nursing intervention (1 month,4 times) and the control group without strong concept of nursing intervention.The two groups were assessed with Hirth hope index (HHI) at discharge and six months after discharge.Results There was no significant difference in hope level between the two groups (P ＞ 0.05)at discharge.Six months after discharge,the scores of hope in the observation group were significantly higher than those in the control group (P ＜ 0.05) and with out statistical sigficance when comaparing to who at discharge(P ＞ 0.05).The HHI score of the control group 6 moths after discharge was significantly lower than that of the control group at discharge (P ＜ 0.05).Conclusion The strong concept nursing intervention during hospitalization can effectively improve the hope level of patients with breast cancer surgery.

The significance of peripheral blood natural killer cell surface CD107a, NKG2D and serum sMICA in patients with extranodal NK/T cell lymphoma, nasal type / 中华内科杂志

The expression of CD107a,NKG2D on the surface of natural killer (NK) cells and serum soluble major histocompatibility complex class Ⅰ chain-related A (sMICA) level in patients with extranodal NK/T cell lymphoma,nasal type (ENKL) were detected.We found that CD107a expression was reduced on the surface of NK cells,suggesting impaired NK cell activity in ENKL patients,which may result from decreased NKG2D expression.

The research of influence of different additional filtration to image quality and radiation dose in the whole brain DSA / 中华放射学杂志

Objective To investigate the effect of different additional filtration thickness of DSA on image quality and radiation dose with cerebral angiography. Methods Prospective collected 90 patients with DSA examination of the whole cerebral artery, patients were divided into A, B and C group according to the time of the examination, each group included 30 cases. Patients underwent conventional DSA, the additional filtration of group A, B and C were (1.0 mmAl+0.1 mmCu), (1.0 mmAl+0.4 mmCu) and (1.0 mmAl+0.9 mmCu), respectively. Dose area product (DAP), air kerma (AK), tube current and tube voltage of anteroposterior and lateral radiography of the whole brain were recorded, and scored the image quality. Eye lens organ dose values were obtained by using simulation phantom and LiF dosemeter under A, B and C groups with three different additional filtrations for cerebral angiography. The image quality scores and the radiation dosewere analyzed by one-way ANOVA tests or Kruskal-Wallis tests. Results The image quality comprehensive score of three groups showed significant difference (F=40.07,P<0. 01), which were (3.8±0.4), (3.6 ± 0.5) and (3.0 ± 0.6), respectively. The DAP and AK value of anteroposterior and lateral radiography of three groups also showed significant difference (P<0.05), B and C group were lower than the A group. Left and right eye lens organ dose were decreased along with the increase of the additional filtration thickness, and the difference between the 3 groups also had significant difference (P<0.01). Conclusion Both the image quality and radiation dose can acquire when conducted the whole brain DSA with 1.0 mmAl+0.4 mmCu additional filtration.

Comparative study of the clinical effect of locking plate and anatomical plate in the treatment of Pilon fracture / 中国基层医药

Objective To compare the clinical effect of two kinds of internal fixation of locking plate and anatomical plate in the treatment of Pilon fracture.Methods 64 patients with Pilon fracture undergoing elective surgery were randomly divided into study group and control group by digital table method,32 cases in each group.The study group received locking plate internal fixation for the treatment,the control group was treated with anatomical plate internal fixation.The clinical efficacy and safety of two kinds of internal fixation methods were compared.Results After treatment,the excellent rate of ankle joint function in the study group(96.88%)was significantly higher than that in the control group (81.25%),and the difference had statistical ssignificance (χ2 =4.01,P 0.05).Conclusion Locking plate fixation in the treatment of Pilon fractures could significantly promote the recovery of joint function,and has the advantages of short operation time,fast fracture healing,less complications and other advantages,it is better than anatomical plate internal fixation.

Effect of cell loss in the CA3 area on hippocampal neurogenesis in chronically epileptic mice / 西安交通大学学报(医学版)

Objective To study the influence of cell loss in the CA3 area on hippocampal neurogenesis in mice with temporal lobe epilepsy .Methods Newly born cells in the subgranular zone (SGZ ) of dentate gyrus were labeled by the proliferation marker bromodeoxyuridine (BrdU ) at 2 months after pilocarpine‐induced status epilepticus .Double labeling of BrdU+ NeuN was carried out to compare neuronal differentiation between type Ⅰand Ⅱ mice ,which represented partial cell loss and almost complete cell loss in the CA 3 area ,respectively .DCX and BrdU single staining were made to analyze the proliferation of progenitor cells in SGZ and the survival of the newly born cells .Results When compared with that of the type Ⅱ mice ,the number of double labeled cells of BrdU+NeuN in the subgranular zone‐granule cell layer (SGZ‐GCL) in the type Ⅰ mice increased significantly ( P0 .05) .No significant difference in the number of DCX + cells in SGZ was found between the typeⅠ and Ⅱ mice (P>0 .05) .At 6 weeks after BrdU labeling ,more remaining BrdU+ cells were found in the SGZ‐GCL in the type Ⅰ mice when compared with their counterparts in the type Ⅱ mice (P<0 .05) .Conclusion The cell loss in the CA3 area of pilocarpine‐induced epileptic mice contributes to the declined hippocampal neurogenesis by exerting negative effects on the survival of newly born cells .

Clinical study on pulmonary protective effect of large dose of ambroxol hydrochloride injection in postoperative patients with severe hypertensive intracerebral hemorrhage / 中国医师进修杂志

Objective To study the pulmonary protective effect of large dose of ambroxol hydrochloride injection in postoperative patients with severe hypertensive intracerebral hemorrhage.Methods One hundred and sixty postoperative patients with hypertensive intracerebral hemorrhage were selected,and the patients were divided into conventional dose group and large dose group by random digits table method with 80 cases each.The therapeutic effect,partial pressure of oxygen,oxygenation index,acute physiology and chronic health evaluation Ⅱ score,antibiotic application time and hospitalization period in intensive care unit were compared between 2 groups.Results After treatment,the partial pressure of oxygen and oxygenation index in large dose group were significantly better than those in conventional dose group:(94.7 ± 7.2) mmHg (1 mmHg =0.133 kPa) vs.(86.5 ± 8.1) mmHg and (420.3 ± 35.5) mmHg vs.(356.0 ± 28.7) mmHg,the acute physiology and chronic health evaluation Ⅱ score was significantly lower than that in conventional dose group:(8.2 ± 3.4) scores vs.(11.6 ± 3.5) scores,the antibiotic application time and hospitalization period in intensive care unit were significantly shorter than those in conventional dose group:(8.5 ± 3.7) d vs.(13.4 ± 5.8) d and (7.3 ± 2.5) d vs.(9.7 ± 5.2) d,and there were statistical differences (P＜ 0.05).The total effective rate in large dose group was significantly higher than that in conventional dose group:92.5％ (74/80) vs.83.8％ (67/80),and there was statistical difference (P ＜ 0.05).Conclusion The pulmonary protective effect of large dose of ambroxol hydrochloride injection is significant in postoperative patients with hypertensive intracerebral hemorrhage.